Capto Blue medium's excellent chemical stability also ensures tolerance for sodium hydroxide, enabling repeated cleaning-in-place (CIP) procedures to remove tightly bound impurities and maintain capacity.Ĭapto Blue medium, an excellent choice for the removal or purification of proteins at both laboratory and process scales, is available as an easy-to use prepacked HiScreen column for fast small-scale purification and process development, as well as in laboratory and process bulk pack sizes. These improvements allow faster flow rates and larger sample volumes, leading to higher throughput and improved process economy.ĭepending on the target molecule, the ligand functionality of Capto Blue may be modified through the use of appropriate buffer salts and buffer conductivity to increase selectivity for desired targets. Size exclusion chromatography resins are not covered in this guide to modern resins. Developed from Blue Sepharose 6 Fast Flow, Capto Blue medium is more chemically stable and has a more rigid agarose base matrix than its predecessor. KTA, BioProcess, Capto, HiTrap, HiScale, HiScreen, MabSelect. We designed resins with optimum pore size and arm space through computer simulation technology and analyzing hydraulic size of different types of biomacromolecules, achieving high dynamic binding capacity and adsorption and desorption in fast mass transfer.īuffer : Buffer A: 20 mM Tris/HC1, pH 8.0 ; Buffer B: 20 mM Tris/HC1+1M NaCI, pH 8.GE Healthcare's Capto Blue affinity chromatography medium is for the capture of human serum albumin (HSA), as well as purification of HSA fusion proteins, blood coagulation factors, enzymes, and recombinant proteins at laboratory and process scales. flowrate for HPS strong cation exchange resinīogen uses multi-scale micro-nano structure control technology to produce chromatography resin with high specific surface area porous structure. Under increased flowrate or column height, the pressure change is relatively smooth, and much lower than agarose-based resin, which is desired for scale-up production processes. Comparing to agarose resins, it provides higher resolution under same process conditions.īesides high resolution, the chromatography resin from Bogen provides ideal pressure-flowrate curve, which helps to maintain low back pressure during scale-up. Capto Butyl HIC resin combines high capacity, high flow rate, and low. GP series : use for purification of antigens of vaccine and virus-based productsīogen uses a series of patented technologies to optimize the particle size, pore structure and micro-surface physical and chemical properties of polymer hard gel, achieving higher basic resolution for resin itself. HP series : use for purification of peptide and recombinant proteinsĬP series : use for purification of recombinant proteins and antibodies Loading capacity 60-120mg/ml (depends on application) And please contact Bogen for more supports, if you need other chromatography resin products with non-standard particle sizes. The hard gel chromatography resin developed by Bogen (A Duoning company) uses high-strength hydrophilic polyacrylate beads as matrix, and unique flexible hydrophilic modification technology to further eliminate the non-specific binding caused by the strong hydrophobic micro-surface of chromatography resins based on hard gel, which increases the recovery of active protein, improves the robustness and repeatability of process.īogen provides wide range of particle sizes for different purification process stages: for capture or crude feed purification, we recommend chromatography resin products with average particle size of 70 μm or 120 μm, for high-viscosity feed, consider resins with particle size of 120 μm or 200 μm for polishing step, we recommend resins with average particle size of 40 μm or 70 μm. Ion exchange resin is one of the most commonly used protein purification methods, which can separate target protein from impurities based on the difference in the isoelectric point of the proteins.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |